Nguyen Xuan Huy
Biography
Dr. Nguyen Xuan Huy is an Associate Professor and Deputy Director of the Department of Science, Technology, and International Relations at Hue University, Vietnam, with expertise in biotechnology. He earned an M.Sc. (2006) and a Ph.D. (2012) in biotechnology from Jeonbuk National University (formerly Chonbuk National University), South Korea, and conducted postdoctoral research at Jeonbuk National University and Ghent University, Belgium. His research focuses on plant-based vaccines, recombinant protein production, transgenic plants for disease resistance, and biotechnology applications in aquaculture. Dr. Huy has led multiple research projects funded by prestigious organizations as published more than 30 papers indexed in Scopus and Web of Science. His work has advanced vaccine development and genetic engineering, contributing to sustainable agriculture and aquaculture. As an academic leader, he also plays a key role in educational quality accreditation, fostering innovation and international collaboration in biotechnology.
Research Interest
University of Agriculture and Forestry
Abstract
Codon-Optimized LvCTL3 Expression to Enhance Disease Resistance in Litopenaeus vannamei
C-type lectins (CTLs) are critical components of the shrimp innate immune system, contributing to pathogen recognition, immune activation and microbial homeostasis. Among these, LvCTL3, a key C-type lectin in the whiteleg shrimp (Litopenaeus vannamei), is highly expressed in immune-related tissues, including the hepatopancreas and hemocytes. LvCTL3 supports innate immunity by recognizing pathogens, agglutinating bacteria (e.g., Vibrio spp., Bacillus subtilis), and aiding in the clearance of bacteria and viruses, such as white spot syndrome virus. In this study, the codon-optimized sequence of LvCTL3 for Escherichia coli expression was synthesized, cloned and expressed in E. coli BL21 (DE3) using the pCold DNA cold-shock expression system. High-level expression of the LvCTL3 protein was confirmed through SDS-PAGE and Western blot analysis with Anti-His Tag antibodies. The purified recombinant LvCTL3 protein was incorporated into basal diets to evaluate its effects on immune parameters, growth performance, and disease resistance in L. vannamei against Acute Hepatopancreatic Necrosis Disease causing Vibrio parahaemolyticus. Successful functional expression of LvCTL3 demonstrates its potential as a dietary supplement to enhance disease resistance in industrial shrimp aquaculture.
