Madhu PC
Biography
I am a Committed and Hardworking Individual who enjoy Challenges. I have a clear, logical mind with a Practical Approach to Achieved my carrier goals. I have an avid curiosity for delving deep into subjects and this have Motivated me to pursue a Biotechnologist. I am confident in my ability to produce and while I prepare for the worst, I do the work necessary to tilt the odds so that the best will happen.University of Genova, Italy in the year 2010. Thesis entitled “Glycosylation in NCLDVs: L-rhamnose biosynthetic pathway in ATCV-1, Mimivirus and sugar composition of the Chlorella viruses”, supervised by Prof. Michela Tonetti, PhD, MD, Professor of Biochemistry. Department of Experimental Medicine (DIMES) University of Genova, Italy. MAHATMAGANDI UNIVERSITY KOTTAYAM KERALA MASTER OF SCIENCE -BIOTECHNOLOGY SECOND CLASS 1999 - 2001 BACHLOR OF SCIENCE – ZOOLOGY FIRST CLASS 1996 - 1999
Research Interest
Abstract
Dairy Effluent a Sustainable Production of food Grade Enzymes and Irrigation water Madhu P C, MET’S School of Engineering, Kerala-680732, India.
Abstract
Utilization and Treatment of dairy Industry waste water play a pivotal role in managing the Wastewater Generated during Various Operations in the dairy industry. These Treatment are to remove harmful Contaminants and pollutants from the wastewater before it is discharged into the near by freshwater bodies. Proteases are proteyolytic enzymes having application in baking, food processing, protein modification etc. As a commodity product, pressure on protease market is on prize reduction and increasing performance. There are three types of industrial protease such as Acid protease, Alkaline protease and Nutral protease are the most utilizing proteins among the three enzymes. Objective: The main objective of the present study was to isolate a potent protease-producing microorganism and formulate a cost effective medium for neutral protease synthesis by the potent microbial culture thus the remaining waste water can be utilized for irrigation purpose. Result and Conclusion: In order to achieve the objective, a proteolytic bacterium was isolated from soil using milk agar medium and the bacteria was identified as Bacillus sp. by morphological and biochemical characterization. Dairy industry effluent was then studied as a medium for neutral protease synthesis by the potent bacteria. Supplementation of mineral salt to the medium did not show profound influence of environmental factors such as medium pH, incubation temperature, agitation rate and incubation time on enzyme production. Optimum enzyme titers were found at pH7 when incubated at 37°C and 120 rpm 48 h. Dairy industry effluent was thus found to be a cost effective medium for neutral protease synthesis by Bacillus sp. After the treatment the produced waste water can be used for irrigation purposes.
Keywords: Dairy effluent, Milk agar, Bacillus sp., Neutral protease, Irrigation, Agriculture.
